The company has confirmed it will no longer sell the EOS-1v, as reported by PetaPixel.
Canon said in a statement on its website: "Thank you very much for your continued patronage of Canon products. By the way, we are finally decided to end sales for the film single lens reflex camera EOS-1v."
The company actually ceased production of the EOS-1v in 2010 but continued to sell the remaining stock.
The professional 35mm camera was launched as part of Canon's fifth generation models in 2000 and its body became the model for future cameras.
Canon said it will continue to repair existing EOS-1v's until October 31st 2025.
This move does not mark the death of film cameras just yet.
Nikon still sells two fil camera - the F6 and the FM10.
Photographic film has seen something of a revival in recent years.
In 2017 both Film Ferrania and Kodak announced the relaunch of 35mm film which it had previously discontinued.
Fujifilm's instant film "Instax" cameras are also still popular.